The plant organellar primase-helicase directs template recognition and primosome assembly via its zinc finger domain.

ABSTRACT:

Background

The mechanisms and regulation for DNA replication in plant organelles are largely unknown, as few proteins involved in replisome assembly have been biochemically studied. A primase-helicase dubbed Twinkle (T7 gp4-like protein with intramitochondrial nucleoid localization) unwinds double-stranded DNA in metazoan mitochondria and plant organelles. Twinkle in plants is a bifunctional enzyme with an active primase module. This contrast with animal Twinkle in which the primase module is inactive. The organellar primase-helicase of Arabidopsis thaliana (AtTwinkle) harbors a primase module (AtPrimase) that consists of an RNA polymerase domain (RPD) and a Zn + + finger domain (ZFD).

Results

Herein, we investigate the mechanisms by which AtTwinkle recognizes its templating

SUBMITTER: Peralta-Castro A 

PROVIDER: S-EPMC10557236 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature