Neural stem/progenitor cells from adult canine cervical spinal cord have the potential to differentiate into neural lineage cells.

ABSTRACT:

Background

• Neural stem/progenitor cells (NSPCs) are multipotent self-renewing cells that can be isolated from the brain or spinal cord. As they need to be isolated from neural tissues, it is difficult to study human NSPCs. To facilitate NSPC research, we attempted to isolate NSPCs from dogs, as dogs share the environment and having many similar diseases with humans. We collected and established primary cultures of ependymal and subependymal cells from the central canal of the cervical spinal cord of adult dogs. To isolate pure NSPCs, we employed the monolayer culture and selective medium culture methods. We further tested the ability of the NSPCs to form neurospheres (using the suspension culture method) and evaluated their differentiation potential.

Results

• The cells ha

SUBMITTER: Kim WK 

PROVIDER: S-EPMC10557334 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature