A fast and inexpensive genotyping system for the simultaneous analysis of human and Aedes albopictus short tandem repeats.

ABSTRACT:

Background

Determination of the interactions between hematophagous mosquitoes and their human hosts is of great importance for better understanding the transmission dynamics of mosquito-borne arboviruses and developing effective strategies to mitigate risk. Genetic analysis of human and mosquito DNA can play a key role in this, but commercial kits for human short tandem repeat (STR) genotyping are expensive and do not allow for the simultaneous STR analysis of host and vector DNA. Here, we present an inexpensive and straightforward STR-loci multiplex system capable of simultaneously amplifying Aedes albopictus and human STRs from blood-fed mosquitoes. Additionally, we examine the effect of storage methods and post-feeding time on the integrity of host DNA.

Methods

Thirty-fiv

SUBMITTER: Albo Timor A 

PROVIDER: S-EPMC10557338 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature