Isomer-Resolved Mass Spectrometry Imaging of Acidic Phospholipids.

ABSTRACT: The biological functions of lipids are entirely dependent on their molecular structures with even small changes in structure─such as different sites of unsaturation─providing critical markers for changes in the underlying metabolism. Conventional mass spectrometry imaging (MSI) approaches, however, face the twin challenges of mixture and structural complexity and are typically unable to differentiate lipid isomers that differ only in the position(s) of carbon-carbon double bonds. Recent coupling of ozone-induced dissociation (OzID) with matrix-assisted laser desorption/ionization (MALDI)-MSI has demonstrated the potential to map changes in individual double-bond isomers, thus enabling visualization of the modulation in lipid desaturation in adjacent tissue types. This has, to date, only be

SUBMITTER: Claes BSR 

PROVIDER: S-EPMC10557375 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature