Optimizing CRE and PhiC31 mediated recombination in Aedes aegypti.

ABSTRACT: Introduction: Genetic manipulation of Aedes aegypti is key to developing a deeper understanding of this insects' biology, vector-virus interactions and makes future genetic control strategies possible. Despite some advances, this process remains laborious and requires highly skilled researchers and specialist equipment. Methods: Here we present two improved methods for genetic manipulation in this species. Use of transgenic lines which express Cre recombinase and a plasmid-based method for expressing PhiC31 when injected into early embryos. Results: Use of transgenic lines which express Cre recombinase allowed, by simple crossing schemes, germline or somatic recombination of transgenes, which could be utilized for numerous genetic manipulations. PhiC31 integrase

SUBMITTER: Carabajal Paladino LZ 

PROVIDER: S-EPMC10557486 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature