Calorimetric analysis using DNA thermal stability to determine protein concentration.

ABSTRACT: It was recently reported for two globular proteins and a short DNA hairpin in NaCl buffer that values of the transition heat capacities, C_p,DNA and C_p,PRO, for equal concentrations (mg/mL) of DNA and proteins, are essentially equivalent (differ by less than 1%). Additional evidence for this equivalence is presented that reveals this phenomenon does not depend on DNA sequence, buffer salt, or T_m. Sequences of two DNA hairpins were designed to confer a near 20°C difference in their T_m's. For the molecules, in NaCl and CsCl buffer the evaluated C_p,PRO and C_p,DNA were equivalent. Based on the equivalence of transition heat capacities, a calorimetric method was devised to de

SUBMITTER: Eskew MW 

PROVIDER: S-EPMC10557601 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature