Multimodal delineation of a layer of effector function among exhausted CD8 T cells in tumors.

ABSTRACT: The anti-tumor function of CD8 T cells is limited through well-established pathways of T cell exhaustion (T_EX). Strategies to capture emergent functional states amongst this dominant trajectory of dysfunction are necessary to find pathways to durable anti-tumor immunity. By leveraging transcriptional reporting (by the fluorescent protein TFP) of the T cell activation marker Cd69, related to upstream AP-1 transcription factors, we define a classifier for potent versus suboptimal CD69+ activation states arising from T cell stimulation. In tumors, this delineation acts an additional functional readout along the T_EX differentiation trajectory, within and across T_EX subsets, marked by enhanced effector cytokine and granzyme B production. The more potent s

SUBMITTER: Ray A 

PROVIDER: S-EPMC10557647 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature