Differential regulation by CD47 and thrombospondin-1 of extramedullary erythropoiesis in mouse spleen.

ABSTRACT: Extramedullary erythropoiesis is not expected in healthy adult mice, but erythropoietic gene expression was elevated in lineage-depleted spleen cells from cd47^-/- mice. Expression of several genes associated with early stages of erythropoiesis was elevated in mice lacking CD47 or its signaling ligand thrombospondin-1, consistent with previous evidence that this signaling pathway inhibits expression of multipotent stem cell transcription factors in spleen. In contrast, cells expressing markers of committed erythroid progenitors were more abundant in cd47^-/- spleens but significantly depleted in thbs1^-/- spleens. Single cell transcriptome and flow cytometry analyses indicated that loss of CD47 is associated with accumulation and increased

SUBMITTER: Banerjee R 

PROVIDER: S-EPMC10557659 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature