HaloTag display enables quantitative single-particle characterization and functionalization of engineered extracellular vesicles.

ABSTRACT: Extracellular vesicles (EVs) play key roles in diverse biological processes, transport biomolecules between cells, and have been engineered for therapeutic applications. A useful EV bioengineering strategy is to express engineered proteins on the EV surface to confer targeting, bioactivity, and other properties. Measuring how incorporation varies across a population of EVs is important for characterizing such materials and understanding their function, yet it remains challenging to quantitatively characterize the absolute number of engineered proteins incorporated at single-EV resolution. To address these needs, we developed a HaloTag-based characterization platform in which dyes or other synthetic species can be covalently and stoichiometrically attached to engineered proteins on the EV s

SUBMITTER: Mitrut RE 

PROVIDER: S-EPMC10557717 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature