Genome-wide CRISPR activation screen identifies JADE3 as an antiviral activator of NF-kB.

ABSTRACT: The innate immune system features a web of interacting pathways that require exquisite regulation. To identify novel nodes in this immune landscape we conducted a gain of function, genome-wide CRISPR activation screen with influenza A virus. We identified both appreciated and novel antiviral genes, including JADE3 a protein involved in directing the histone acetyltransferase HBO1 complex to modify chromatin and regulate transcription. JADE3 is both necessary and sufficient to restrict influenza A virus infection. Interestingly, expression of the closely related paralogues JADE1 and JADE2 are unable to restrict influenza A virus infection, suggesting a distinct function of JADE3. We identify both shared and unique transcriptional signatures between uninfected cells expressing JADE3 and JADE

SUBMITTER: Munir M 

PROVIDER: S-EPMC10557722 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature