Evidence for a role of human blood-borne factors in mediating age-associated changes in molecular circadian rhythms.

ABSTRACT: Aging is associated with a number of physiologic changes including perturbed circadian rhythms; however, mechanisms by which rhythms are altered remain unknown. To test the idea that circulating factors mediate age-dependent changes in peripheral rhythms, we compared the ability of human serum from young and old individuals to synchronize circadian rhythms in culture. We collected blood from apparently healthy young (age 25-30) and old (age 70-76) individuals at 14:00 and used the serum to synchronize cultured fibroblasts. We found that young and old sera are equally competent at initiating robust ~24h oscillations of a luciferase reporter driven by clock gene promoter. However, cyclic gene expression is affected, such that young and old sera promote cycling of different sets of genes. Gen

SUBMITTER: Schwarz JE 

PROVIDER: S-EPMC10557775 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature