Production of Highly Uniform Midbrain Organoids from Human Pluripotent Stem Cells.

ABSTRACT: Brain organoids have been considered as an advanced platform for in vitro disease modeling and drug screening, but numerous roadblocks exist, such as lack of large-scale production technology and lengthy protocols with multiple manipulation steps, impeding the industrial translation of brain organoid technology. Here, we describe the high-speed and large-scale production of midbrain organoids using a high-throughput screening-compatible platform within 30 days. Micro midbrain organoids (µMOs) exhibit a highly uniform morphology and gene expression pattern with minimal variability. Notably, µMOs show dramatically accelerated maturation, resulting in the generation of functional µMOs within only 30 days of differentiation. Furthermore, individual µMOs displ

SUBMITTER: Yao X 

PROVIDER: S-EPMC10558263 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature