Integrative analysis of cancer-associated fibroblast signature in gastric cancer.

ABSTRACT:

Background

CAFs regulate the signaling of GC cells by promoting their migration, invasion, and proliferation and the function of immune cells as well as their location and migration in the TME by remodeling the extracellular matrix (ECM). This study explored the understanding of the heterogeneity of CAFs in TME and laid the groundwork for GC biomarker and precision treatment development.

Methods

The scRNA-seq and bulk RNA-seq datasets were obtained from GEO and TCGA. The prognostic significance of various CAFs subtypes was investigated using ssGSEA combined with Kaplan-Meier analysis. POSTN expression in GC tissues and CAFs was detected using immunohistochemistry, immunofluorescence, and Western blotting. Differential expression analysis identified the differentially express

SUBMITTER: Zhao Z 

PROVIDER: S-EPMC10558323 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature