Fe₃O₄@SiO₂@NiAl-LDH microspheres implication in separation, kinetic and structural properties of phenylalanine dehydrogenase.

ABSTRACT: Fe₃O₄@SiO₂@NiAl-LDH three-components microsphere contains a Fe3O4@SiO2 magnetic core and a layered double hydroxide with nickel cation provide the binding ability to (His)-tagged-protein and exhibits high performance in protein separation and purification. The morphology and chemistry of the synthesized Fe₃O₄@SiO₂@NiAl-LDH microspheres were characterized by energy-dispersive X-ray spectroscopy (EDX), scanning electron microscopy (SEM), X-ray diffraction (XRD), Fourier transform infrared (FTIR), vibrating sample magnetometer (VSM), Dynamic light scattering (DLS). Purified enzyme was assesed with SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis and intrinsic fluorescence spectroscopy. In this study, the sepa

SUBMITTER: Amirahmadi M 

PROVIDER: S-EPMC10558515 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature