Single-cell transcriptome profiling reveals immune and stromal cell heterogeneity in primary Sjogren's syndrome.

ABSTRACT: Primary Sjögren's syndrome (pSS) is a complex autoimmune disease characterized by lymphocytic infiltration and exocrine dysfunction, particularly affecting the salivary gland (SG). We employed single-cell RNA sequencing to investigate cellular heterogeneity in 11 patients with pSS and 5 non-SS controls. Notably, patients with pSS exhibited downregulated SOX9 in myoepithelial cells, potentially associated with impaired epithelial regeneration. An expanded ACKR1⁺ endothelial subpopulation in patients with pSS suggested a role in facilitating lymphocyte transendothelial migration. Our analysis of immune cells revealed expanded IGHD⁺ naive B cells in peripheral blood from patients with pSS. Pseudotime trajectory analysis outlined a bifurcated differentiation pathway for p

SUBMITTER: Xiang N 

PROVIDER: S-EPMC10558796 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature