Generation of 3D lung organoids from human induced pluripotent stem cells for modeling of lung development and viral infection.
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ABSTRACT:
SUBMITTER: Tiwari SK
PROVIDER: S-EPMC10558843 | biostudies-literature | 2023 Sep
REPOSITORIES: biostudies-literature
Publications
Generation of 3D lung organoids from human induced pluripotent stem cells for modeling of lung development and viral infection.
Tiwari Shashi Kant SK Rana Tariq M TM
Heliyon 20230829 9
The lack of physiologically relevant <i>in vitro</i> models has hampered progress in understanding human lung development and disease. Here, we describe a protocol in which human induced pluripotent stem cells (hiPSCs) undergo stepwise differentiation into definitive endoderm (>88% population) to three-dimensional (3D) lung organoids (LORGs), which contain both epithelial and mesenchymal cellular architecture and display proximal and distal airway patterning. These LORGs can maintained for more ...[more]
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