Restriction-ligation-independent production of a TVCV infectious clone and a TVCV-based gene expression vector.

ABSTRACT: Transgenic expression of proteins in plants is central to research and biotechnology, and, often, it is desirable to obtain this expression without altering the nuclear or plastid genomes. Thus, expression vectors based on plant viruses that infect multiple cells are useful; furthermore, they are also advantageous for studies of the life cycle of the virus itself. Here, we report the development of an expression vector based on a Turnip vein-clearing virus (TVCV), a tobamovirus known to easily infect two model plants, Nicotiana benthamiana, and Arabidopsis thaliana. Avoiding restriction digestion, we utilized a restriction-ligation-independent cloning approach to construct an infectious cDNA clone of TVCV from the viral RNA and then to convert this clone to a gene expr

SUBMITTER: Mirauti A 

PROVIDER: S-EPMC10559237 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature