Muscarinic receptor agonist-induced βPix binding to β-catenin promotes colon neoplasia.

ABSTRACT: M₃ muscarinic receptors (M₃R) modulate β-catenin signaling and colon neoplasia. CDC42/RAC guanine nucleotide exchange factor, βPix, binds to β-catenin in colon cancer cells, augmenting β-catenin transcriptional activity. Using in silico, in vitro, and in vivo approaches, we explored whether these actions are regulated by M₃R. At the invasive fronts of murine and human colon cancers, we detected co-localized nuclear expression of βPix and β-catenin in stem cells overexpressing M₃R. Using immunohistochemistry, immunoprecipitation, proximity ligand, and fluorescent cell sorting assays in human tissues and established and primary human colon cancer cell cultures, we detected time-dependent M₃R agonist-induced cytoplasmic and nuclear associ

SUBMITTER: Cheng K 

PROVIDER: S-EPMC10560271 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature