Improvement of ethanol and 2,3-butanediol production in Saccharomyces cerevisiae by ATP wasting.

ABSTRACT:

Background

"ATP wasting" has been observed in ¹³C metabolic flux analyses of Saccharomyces cerevisiae, a yeast strain commonly used to produce ethanol. Some strains of S. cerevisiae, such as the sake strain Kyokai 7, consume approximately two-fold as much ATP as laboratory strains. Increased ATP consumption may be linked to the production of ethanol, which helps regenerate ATP.

Results

This study was conducted to enhance ethanol and 2,3-butanediol (2,3-BDO) production in the S. cerevisiae strains, ethanol-producing strain BY318 and 2,3-BDO-producing strain YHI030, by expressing the fructose-1,6-bisphosphatase (FBPase) and ATP synthase (ATPase) genes to induce ATP dissipation. The introduction of a futile cycle for ATP consumption in the pathway was achieved by exp

SUBMITTER: Yatabe F 

PROVIDER: S-EPMC10560415 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature