Single-cell transcriptomics identifies new blood cell populations in Drosophila released at the onset of metamorphosis.

ABSTRACT: Drosophila blood cells called hemocytes form an efficient barrier against infections and tissue damage. During metamorphosis, hemocytes undergo tremendous changes in their shape and behavior, preparing them for tissue clearance. Yet, the diversity and functional plasticity of pupal blood cells have not been explored. Here, we combine single-cell transcriptomics and high-resolution microscopy to dissect the heterogeneity and plasticity of pupal hemocytes. We identified undifferentiated and specified hemocytes with different molecular signatures associated with distinct functions such as antimicrobial, antifungal immune defense, cell adhesion or secretion. Strikingly, we identified a highly migratory and immune-responsive pupal cell population expressing typical markers of the posterior sign

SUBMITTER: Hirschhauser A 

PROVIDER: S-EPMC10560556 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature