A novel design for magnetic tweezers with wide-range temperature control.

ABSTRACT: Single-molecule manipulation technologies have proven to be powerful tools for studying the molecular mechanisms and physical principles underlying many essential biological processes. However, achieving wide-range temperature control has been challenging due to thermal drift that undermines the stability of the instrument. This limitation has made it difficult to study biomolecules from thermophiles at their physiologically relevant temperatures and has also hindered the convenient measurement of temperature-sensitive biomolecular interactions and the fundamental thermodynamic properties of biomolecules. In this work, we present a novel design of magnetic tweezers that uses a reflective coverslip and dry objective lens to insulate the heat conductance between the sample and the objective

SUBMITTER: Zhou Y 

PROVIDER: S-EPMC10560670 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature