Collective heterogeneity of mitochondrial potential in contact inhibition of proliferation.

ABSTRACT: In the epithelium, cell density and cell proliferation are closely connected to each other through contact inhibition of proliferation (CIP). Depending on cell density, CIP proceeds through three distinct stages: the free-growing stage at low density, the pre-epithelial transition stage at medium density, and the post-epithelial transition stage at high density. Previous studies have elucidated how cell morphology, motion, and mechanics vary in these stages. However, it remains unknown whether cellular metabolism also has a density-dependent behavior. By measuring the mitochondrial membrane potential at different cell densities, here we reveal a heterogeneous landscape of metabolism in the epithelium, which appears qualitatively distinct in three stages of CIP and did not follow the trend

SUBMITTER: Thurakkal B 

PROVIDER: S-EPMC10560682 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature