Novel method for prediction of combinatorial phase-variable gene expression states.

ABSTRACT: Short sequence repeat mediated phase variation results in diverse phenotype presentation in many bacteria including Campylobacter and Neisseria species. Current methods for identifying the expression states of phase-variable genes involve taking a high number of single colonies. This approach is subject to bias, sampling effects and high workloads that reduce the ability to perform intermediary sampling. The use of high concentration colony sweeps provides a work around but reduces the resolution of combinatorial expression profiles (termed phasotypes). A parsimonious approach combining both single colony and sweep data was developed to overcome these limitations. The critical methodological advance is the use of an algorithm that utilises the experimental data from the two s

SUBMITTER: Holmes J 

PROVIDER: S-EPMC10561117 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature