A Multipathway Phosphopeptide Standard for Rapid Phosphoproteomics Assay Development.

ABSTRACT: Recent advances in methodology have made phosphopeptide analysis a tractable problem for many proteomics researchers. There are now a wide variety of robust and accessible enrichment strategies to generate phosphoproteomes while free or inexpensive software tools for quantitation and site localization have simplified phosphoproteome analysis workflow tremendously. As a research group under the Association for Biomolecular Resource Facilities umbrella, the Proteomics Standards Research Group has worked to develop a multipathway phosphopeptide standard based on a mixture of heavy-labeled phosphopeptides designed to enable researchers to rapidly develop assays. This mixture contains 131 mass spectrometry vetted phosphopeptides specifically chosen to cover as many known biologically interestin

SUBMITTER: Searle BC 

PROVIDER: S-EPMC10561125 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature