Equine maternal aging affects the metabolomic profile of oocytes and follicular cells during different maturation time points.

ABSTRACT: Introduction: Oocyte quality and fertility decline with advanced maternal age. During maturation within the ovarian follicle, the oocyte relies on the associated somatic cells, specifically cumulus and granulosa cells, to acquire essential components for developmental capacity. Methods: A nontargeted metabolomics approach was used to investigate the effects of mare age on different cell types within the dominant, follicular-phase follicle at three time points during maturation. Metabolomic analyses from single oocytes and associated cumulus and granulosa cells allowed correlations of metabolite abundance among cell types. Results and Discussion: Overall, many of the age-related changes in metabolite abundance point to Impaired mitochondrial metabolic function and oxida

SUBMITTER: Catandi GD 

PROVIDER: S-EPMC10561129 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature