Aging and obesity prime the methylome and transcriptome of adipose stem cells for disease and dysfunction.

ABSTRACT: The epigenome of stem cells occupies a critical interface between genes and environment, serving to regulate expression through modification by intrinsic and extrinsic factors. We hypothesized that aging and obesity, which represent major risk factors for a variety of diseases, synergistically modify the epigenome of adult adipose stem cells (ASCs). Using integrated RNA- and targeted bisulfite-sequencing in murine ASCs from lean and obese mice at 5- and 12-months of age, we identified global DNA hypomethylation with either aging or obesity, and a synergistic effect of aging combined with obesity. The transcriptome of ASCs in lean mice was relatively stable to the effects of age, but this was not true in obese mice. Functional pathway analyses identified a subset of genes with critical role

SUBMITTER: Xie S 

PROVIDER: S-EPMC10561192 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature