The cell cycle protein MAD2 facilitates endocytosis of the serotonin transporter in the neuronal soma.

ABSTRACT: Monoamine transporters retrieve serotonin (SERT), dopamine (DAT), and norepinephrine (NET) from the synaptic cleft. Transporter internalization contributes to the regulation of their surface expression. Clathrin-mediated endocytosis of plasma membrane proteins requires adaptor protein-2 (AP2), which recruits cargo to the nascent clathrin cage. However, the intracellular portions of monoamine transporters are devoid of a conventional AP2-binding site. Here, we identify a MAD2 (mitotic arrest deficient-2) interaction motif in the C-terminus of SERT, which binds the closed conformation of MAD2 and allows for the recruitment of two additional mitotic spindle assembly checkpoint (SAC) proteins, BubR1 and p31^comet , and of AP2. We visualize MAD2, BubR1, and p31^comet in dors

SUBMITTER: Koban F 

PROVIDER: S-EPMC10561363 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature