β-Glucosidase activity and antimicrobial properties of potentially probiotic autochthonous lactic cultures.

ABSTRACT:

Background

The demand for lactic acid bacteria products, especially probiotics, has increased. Bacteria that increase polyphenol bioavailability and act as bio preservatives are sought after. This study aims to identify autochthonous lactic acid cultures from EMBRAPA that demonstrate β-glucosidase activity and inhibitory effect on microbial sanitary indicators.

Methods

Cell-free extracts were obtained by sonicating every 5 s for 40 min. The extracts were mixed with cellobiose and incubated at 50 °C. The reaction was stopped by immersing the tubes in boiling water. The GOD-POD reagent was added for spectrophotometer readings. Antimicrobial activity was tested against reference strains using the agar well diffusion method. Lactic cultures in MRS broth were added to 0.9 cm well

SUBMITTER: Pires de Oliveira Galdino IKC 

PROVIDER: S-EPMC10561641 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature