Extracellular Matrix Influences Gene Expression and Differentiation of Mouse Trophoblast Stem Cells.

ABSTRACT: Trophoblast stem (TS) cells were first isolated from the mouse placenta; however, little is known about their maintenance and niche in vivo. TS cells, like other stem cells, have a unique microenvironment in which the extracellular matrix (ECM) is a component. Placental pathology is associated with ECM change. However, how these changes and the individual ECM components impact the maintenance or differentiation of TS cells has not been established. This study identified which ECM component(s) maintain the greatest expression of markers associated with undifferentiated mouse trophoblast stem (mTS) cells and which alter the profile of markers of differentiation based on mRNA analysis. mTS cells cultured on individual ECM components and subsequent quantitative polymerase chain reaction analys

SUBMITTER: Natale BV 

PROVIDER: S-EPMC10561768 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature