Fully automated sequential immunofluorescence (seqIF) for hyperplex spatial proteomics.

ABSTRACT: Tissues are complex environments where different cell types are in constant interaction with each other and with non-cellular components. Preserving the spatial context during proteomics analyses of tissue samples has become an important objective for different applications, one of the most important being the investigation of the tumor microenvironment. Here, we describe a multiplexed protein biomarker detection method on the COMET instrument, coined sequential ImmunoFluorescence (seqIF). The fully automated method uses successive applications of antibody incubation and elution, and in-situ imaging enabled by an integrated microscope and a microfluidic chip that provides optimized optical access to the sample. We show seqIF data on different sample types such as tumor and healthy tissue,

SUBMITTER: Rivest F 

PROVIDER: S-EPMC10562446 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature