Short-term assays for mesenchymal stromal cell immunosuppression of T-lymphocytes.

ABSTRACT:

Introduction

Trauma patients are susceptible to coagulopathy and dysfunctional immune responses. Mesenchymal stromal cells (MSCs) are at the forefront of the cellular therapy revolution with profound immunomodulatory, regenerative, and therapeutic potential. Routine assays to assess immunomodulation activity examine MSC effects on proliferation of peripheral blood mononuclear cells (PBMCs) and take 3-7 days. Assays that could be done in a shorter period of time would be beneficial to allow more rapid comparison of different MSC donors. The studies presented here focused on assays for MSC suppression of mitogen-stimulated PBMC activation in time frames of 24 h or less.

Methods

Three potential assays were examined-assays of apoptosis focusing on caspase activation, assays of p

SUBMITTER: Herzig MC 

PROVIDER: S-EPMC10562633 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature