Evaluation of a self-amplifying mRNA reporter vaccine in explant models of broiler chickens.

ABSTRACT: In order to minimize animal loss and economical loss, industrial poultry is heavily vaccinated against infectious agents. mRNA vaccination is an effective vaccination platform, yet little to no comprehensive, comparative studies in avians can be found. Nevertheless, poultry mRNA vaccination could prove to be very interesting due to the relatively low production cost, especially true when using self-amplifying mRNA (saRNA), and their extreme adaptability to new pathogens. The latter could be particularly useful when new pathogens join the stage or new variants arise. As a first step toward the investigation of saRNA vaccines in poultry, this study evaluates a luciferase-encoding saRNA in avian tracheal explants, conjunctival explants, primary chicken cecal cells and 18-day embryonated eggs.

SUBMITTER: Snoeck J 

PROVIDER: S-EPMC10562763 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature