[miRNA-128-3p inhibits malignant behavior of glioma cells by downregulating KLHDC8A expression].

ABSTRACT:

Objective

To determine whether miRNA-128-3p regulates malignant biological behavior of glioma cells by targeting KLHDC8A.

Methods

Dual-luciferase reporter assays, qRT-PCR and Western blotting were used to verify the targeting of miRNA-128-3p to KLHDC8A. Edu assay, flow cytometry, Transwell assay and would healing assay were used to determine the effects of changes in miRNA-128-3p and KLHDC8A expression levels on malignant behavior of glioma cells. Rescue experiment was carried out to verify that miRNA-128-3p regulated glioma cell proliferation, apoptosis, invasion and migration by targeting KLHDC8A.

Results

The expression level of KLHDC8A was significantly increased in high-grade glioma tissue and was closely related to a poor survival outcome of the patients. Overexp

SUBMITTER: Yu Z 

PROVIDER: S-EPMC10563106 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature