TMT-based quantitative proteomics reveals the targets of andrographolide on LPS-induced liver injury.

ABSTRACT:

Background

Andrographolide (Andro) is a diterpenoid derived from Andrographis paniculate, which has anti-inflammatory, antibacterial, antiviral and hepatoprotective activities. Gram-negative bacterial infections can cause varying degrees of liver injury in chickens, although Andro has been shown to have a protective effect on the liver, its underlying mechanism of action and effects on liver proteins are not known.

Methods

The toxicity of Andro on the viability of leghorn male hepatoma (LMH) cells at different concentrations and times was analyzed by CCK-8 assays. Alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities in the culture supernatants were measured using an automatic biochemical analyzer to evaluate the protective effect of androscopolide o

SUBMITTER: Ge S 

PROVIDER: S-EPMC10563216 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature