Lineage tracing reveals clonal progenitors and long-term persistence of tumor-specific T cells during immune checkpoint blockade.

ABSTRACT: Paired single-cell RNA and T cell receptor sequencing (scRNA/TCR-seq) has allowed for enhanced resolution of clonal T cell dynamics in cancer. Here, we report a scRNA/TCR-seq analysis of 187,650 T cells from 31 tissue regions, including tumor, adjacent normal tissues, and lymph nodes (LN), from three patients with non-small cell lung cancer after immune checkpoint blockade (ICB). Regions with viable cancer cells are enriched for exhausted CD8⁺ T cells, regulatory CD4⁺ T cells (Treg), and follicular helper CD4⁺ T cells (TFH). Tracking T cell clonotypes across tissues, combined with neoantigen specificity assays, reveals that TFH and tumor-specific exhausted CD8⁺ T cells are clonally linked to TCF7⁺SELL⁺ progenitors in tumor d

SUBMITTER: Pai JA 

PROVIDER: S-EPMC10563767 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature