Gene expression signatures of human senescent corneal and conjunctival epithelial cells.

ABSTRACT:

Purpose

This study aimed to investigate the senescent phenotypes of human corneal and conjunctival epithelial cells.

Methods

We examined cell morphology, senescence-associated β-galactosidase (SA-β-gal) activity, cell proliferation, and expression of senescence markers (p16 and p21). RNA sequencing analysis was conducted to compare gene expression profiles between senescent and non-senescent cells. Finally, the potential involvement of senescent cells in the pathogenesis of ocular surface diseases was investigated.

Results

X-irradiated corneal and conjunctival epithelial cells exhibited typical senescence phenotypes, i.e., flattened morphologies, increased SA-β-gal activity, decreased cell proliferation, and increased expression of senescence markers, p16 and p21. RNA

SUBMITTER: Kitazawa K 

PROVIDER: S-EPMC10564427 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature