The complementary roles of VAMP-2, -3, and -7 in platelet secretion and function.

ABSTRACT: Platelet secretion requires Soluble N-ethylmaleimide Sensitive Attachment Protein Receptors (SNAREs). Vesicle SNAREs/Vesicle-Associated Membrane Proteins (v-SNAREs/VAMPs) on granules and t-SNAREs in plasma membranes mediate granule release. Platelet VAMP heterogeneity has complicated the assessment of how/if each is used and affects hemostasis. To address the importance of VAMP-7 (V7), we analyzed mice with global deletions of V3 and V7 together or platelet-specific deletions of V2, V3, and global deletion of V7. We measured the kinetics of cargo release, and its effects on three injury models to define the context-specific roles of these VAMPs. Loss of V7 minimally affected dense and α granule release but did affect lysosomal release. V3^-/-7^-/- and V2^Δ3

SUBMITTER: Joshi S 

PROVIDER: S-EPMC10564522 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature