Role of tear exosomes in the spread of herpes simplex virus type 1 in recurrent herpes simplex keratitis.

ABSTRACT:

Background

Herpes simplex keratitis (HSK) is the most common but serious infectious keratitis with high recurrence. It is predominantly caused by herpes simplex virus type 1 (HSV-1). The spread mechanism of HSV-1 in HSK is not entirely clear. Multiple publications indicate that exosomes participate in the intercellular communication process during viral infections. However, there is rare evidence that HSV-1 spreads in HSK by exosomal pathway. This study aims to investigate the relationship between the spread of HSV-1 and tear exosomes in recurrent HSK.

Methods

Tear fluids collected from total 59 participants were included in this study. Tear exosomes were isolated by ultracentrifugation, then identified by silver staining and western blot. The size was determined by dynamic

SUBMITTER: Huang H 

PROVIDER: S-EPMC10564740 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature