Calbindin 2-specific deletion of arginase 2 preserves visual function after optic nerve crush.

ABSTRACT: We previously found that global deletion of the mitochondrial enzyme arginase 2 (A2) limits optic nerve crush (ONC)-induced neuronal death. Herein, we examined the cell-specific role of A2 in this pathology by studies using wild type (WT), neuronal-specific calbindin 2 A2 KO (Calb2^cre/+ A2 ^f/f), myeloid-specific A2 KO (LysM^cre/+ A2^f/f), endothelial-specific A2 KO (Cdh5^cre/+ A2^f/f), and floxed controls. We also examined the impact of A2 overexpression on mitochondrial function in retinal neuronal R28 cells. Immunolabeling showed increased A2 expression in ganglion cell layer (GCL) neurons of WT mice within 6 h-post injury and inner retinal neurons after 7 days. Calb2 A2 KO mice showed improved neuronal survival, decreased TUN

SUBMITTER: Zaidi SAH 

PROVIDER: S-EPMC10564748 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature