Performance of a RAD51-based functional HRD test on paraffin-embedded breast cancer tissue.

ABSTRACT:

Purpose

BRCA-deficient breast cancers (BC) are highly sensitive to platinum-based chemotherapy and PARP inhibitors due to their deficiency in the homologous recombination (HR) pathway. However, HR deficiency (HRD) extends beyond BRCA-associated BC, highlighting the need for a sensitive method to enrich for HRD tumors in an alternative way. A promising approach is the use of functional HRD tests which evaluate the HR capability of tumor cells by measuring RAD51 protein accumulation at DNA damage sites. This study aims to evaluate the performance of a functional RAD51-based HRD test for the identification of HRD BC.

Methods

The functional HR status of 63 diagnostic formalin-fixed paraffin-embedded (FFPE) BC samples was determined by applying the RAD51-FFPE test. Samples were s

SUBMITTER: van Wijk LM 

PROVIDER: S-EPMC10564840 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature