His₁-tagged DM or DDM detergent micelles are reversibly conjugated by nickel ions.

ABSTRACT: Specific conjugation of decyl β-D-maltoside (DM) or dodecyl β-D-maltoside (DDM) detergent micelles is accomplished between pH 7.0-8.5 in the presence of an amphiphilic analog of the amino acid histidine, bound to a 10-carbon hydrocarbon chain (His₁-C10) and Ni²⁺ ions. Following addition of 10-15 wt% PEG-6000 as precipitant, phase separation in the form of oil-rich globules (30-600 µm) is observed by light microscopy. Other divalent cations: Zn²⁺, Fe²⁺, Cu²⁺ lead to dark precipitates rather than colorless globules; while Mg²⁺, Ca²⁺ do not promote any phase separation at all. Even in the absence of precipitant, dynamic light scattering (DLS) measurements demonstrate that DM micelles (hydrodynamic size ~ 6 nm) or DDM

SUBMITTER: Lal M 

PROVIDER: S-EPMC10564902 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature