IGF2BP2 acts as a m⁶A modification regulator in laryngeal squamous cell carcinoma through facilitating CDK6 mRNA stabilization.

ABSTRACT: Laryngeal squamous cell carcinoma (LSCC) is one of the most commonly seen cancers in the head and neck region with increasing morbidity and mortality globally. N6-methyladenosine (m⁶A) modification plays a critical role in the carcinogenesis of LSCC. In this study, two datasets from online database were analyzed for differentially expressed genes (DEGs) between LSCC and normal samples. Furthermore, we carried out a series of experiments, including hematoxylin & eosin staining, immunohistochemical (IHC) staining, CCK-8, colony formation, transwell, flow cytometry, xenograft tumor model assays, actinomycin D assay, cycloheximide (CHX) assay, methylated m⁶A RNA immunoprecipitation (Me-RIP), RNA immunoprecipitation (RIP) assay, to verify the relevant findings in vivo and

SUBMITTER: Tang X 

PROVIDER: S-EPMC10564923 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature