Isolation of goat milk small extracellular vesicles by novel combined bio-physical methodology.

ABSTRACT: Introduction: Goat milk is notable as a cost-effective source of exosomes, also known as small extracellular vesicles (sEVs). These nanoparticle-like structures are naturally secreted by cells and have emerged as potential diagnostic agents and drug delivery systems, also supported by their proven therapeutic effects. However, the complexity of goat milk and the lack of standardized protocols make it difficult to isolate pure sEVs. This work presents an optimized approach that combines well-established physical isolation methods with the biological treatment of milk with rennet. Methods: sEVs derived from goat milk were purified using a methodology that combines differential ultracentrifugation, rennet, and size-exclusion chromatography. This novel strategy was compared with

SUBMITTER: Gonzalez MI 

PROVIDER: S-EPMC10564981 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature