Human pluripotent stem cell-derived inner ear organoids recapitulate otic development in vitro.

ABSTRACT: Our molecular understanding of the early stages of human inner ear development has been limited by the difficulty in accessing fetal samples at early gestational stages. As an alternative, previous studies have shown that inner ear morphogenesis can be partially recapitulated using induced pluripotent stem cells directed to differentiate into inner ear organoids (IEOs). Once validated and benchmarked, these systems could represent unique tools to complement and refine our understanding of human otic differentiation and model developmental defects. Here, we provide the first direct comparisons of the early human embryonic otocyst and fetal sensory organs with human IEOs. We use multiplexed immunostaining and single-cell RNA-sequencing to characterize IEOs at three key developmental steps, p

SUBMITTER: Doda D 

PROVIDER: S-EPMC10565253 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature