Identification of optimal conditions for human placental explant culture and extracellular vesicle release.

ABSTRACT: Extracellular vesicles (EVs) can mediate intercellular communication, including signaling between the placenta and maternal tissues. Human placental explant culture is a versatile in vitro model system to investigate placental function. We performed systematic studies in different tissue culture media types and oxygen tensions to identify a defined serum-free culture condition that supports high trophoblast viability and metabolism, as well as the release of similar populations of EVs, compared to traditional undefined conditions that contain media additives potentially contaminated with exogenous EVs. We also determined the time frame in which trophoblast viability and functionality remain optimal. Multiplex vesicle flow cytometry with classical EV and placenta-specific markers rev

SUBMITTER: Tekkatte C 

PROVIDER: S-EPMC10565782 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature