Metabolic engineering and optimization of Escherichia coli co-culture for the de novo synthesis of genkwanin.

ABSTRACT: Genkwanin has various significant roles in nutrition, biomedicine, and pharmaceutical biology. Previously, this compound was chiefly produced by plant-originated extraction or chemical synthesis. However, due to increasing concern and demand for safe food and environmental issues, the biotechnological production of genkwanin and other bioactive compounds based on safe, cheap, and renewable substrates has gained much interest. This paper described recombinant Escherichia coli-based co-culture engineering that was reconstructed for the de novo production of genkwanin from d-glucose. The artificial genkwanin biosynthetic chain was divided into 2 modules in which the upstream strain contained the genes for synthesizing p-coumaric acid from d-glucose, and the downstream module contained a gene

SUBMITTER: Thuan NH 

PROVIDER: S-EPMC10565888 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature