Imprinting at the KBTBD6 locus involves species-specific maternal methylation and monoallelic expression in livestock animals.

ABSTRACT:

Background

The primary differentially methylated regions (DMRs) which are maternally hypermethylated serve as imprinting control regions (ICRs) that drive monoallelic gene expression, and these ICRs have been investigated due to their implications in mammalian development. Although a subset of genes has been identified as imprinted, in-depth comparative approach needs to be developed for identification of species-specific imprinted genes. Here, we examined DNA methylation status and allelic expression at the KBTBD6 locus across species and tissues and explored potential mechanisms of imprinting.

Results

Using whole-genome bisulfite sequencing and RNA-sequencing on parthenogenetic and normal porcine embryos, we identified a maternally hypermethylated DMR between the embryos a

SUBMITTER: Ahn J 

PROVIDER: S-EPMC10565993 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature