Beyond the G protein α subunit: investigating the functional impact of other components of the Gαi₃ heterotrimers.

ABSTRACT:

Background

Specific interactions between G protein-coupled receptors (GPCRs) and G proteins play a key role in mediating signaling events. While there is little doubt regarding receptor preference for Gα subunits, the preferences for specific Gβ and Gγ subunits and the effects of different Gβγ dimer compositions on GPCR signaling are poorly understood. In this study, we aimed to investigate the subcellular localization and functional response of Gαi₃-based heterotrimers with different combinations of Gβ and Gγ subunits.

Methods

Live-cell imaging microscopy and colocalization analysis were used to investigate the subcellular localization of Gαi₃ in combination with Gβ₁ or Gβ₂ heterotrimers, along with representative Gγ subunits. Fur

SUBMITTER: Rysiewicz B 

PROVIDER: S-EPMC10566112 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature