Multi-faceted attributes of salivary cell-free DNA as liquid biopsy biomarkers for gastric cancer detection.

ABSTRACT:

Background

Recent advances in circulating cell-free DNA (cfDNA) analysis from biofluids have opened new avenues for liquid biopsy (LB). However, current cfDNA LB assays are limited by the availability of existing information on established genotypes associated with tumor tissues. Certain cancers present with a limited list of established mutated cfDNA biomarkers, and thus, nonmutated cfDNA characteristics along with alternative biofluids are needed to broaden the available cfDNA targets for cancer detection. Saliva is an intriguing and accessible biofluid that has yet to be fully explored for its clinical utility for cancer detection.

Methods

In this report, we employed a low-coverage single stranded (ss) library NGS pipeline "Broad-Range cell-free DNA-Seq" (BRcfDNA-Seq) usi

SUBMITTER: Swarup N 

PROVIDER: S-EPMC10566128 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature