Post-synthetic benzylation of the mRNA 5' cap via enzymatic cascade reactions.

ABSTRACT: mRNAs are emerging modalities for vaccination and protein replacement therapy. Increasing the amount of protein produced by stabilizing the transcript or enhancing translation without eliciting a strong immune response are major steps towards overcoming the present limitations and improving their therapeutic potential. The 5' cap is a hallmark of mRNAs and non-natural modifications can alter the properties of the entire transcript selectively. Here, we developed a versatile enzymatic cascade for regioselective benzylation of various biomolecules and applied it for post-synthetic modification of mRNA at the 5' cap to demonstrate its potential. Starting from six synthetic methionine analogues bearing (hetero-)benzyl groups, S-adenosyl-l-methionine analogues are formed and utilized for

SUBMITTER: Cornelissen NV 

PROVIDER: S-EPMC10566477 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature